A MICROTUBE CULTURE METHOD FOR MOUSE OOCYTES

Microtube Method for Identification of Neisseria Species

Neisseria species are identified in most diagnostic microbiology laboratories by using carbohydrate degradation methods. Conventional methods require 24 to 48 h of incubation for completion of the test (15). A number of rapid methods which employ various agar bases, growth factors, sugar concentrations, agar concentrations, inoculum sizes, filter paper disks, radiometry, gas chromatography, spe...

In Vitro Maturation, Fertilization and Embryo Culture of Oocytes Obtained from Vitrified Auto-Transplanted Mouse Ovary

Effect of Fetal Mouse Lung Tissue Co-Culture on In Vitro Maturation of Mouse Immature Oocytes

OBJECTIVES The aim of this study was to evaluate the fetal mouse lung tissue co-culture on in vitro maturation (IVM) of mouse immature oocytes. MATERIALS AND METHODS In this experimental study, germinal vesicle (GV) oocytes from ovaries of a group of 25 female mice, 6-8 weeks of age, were dissected after being stimulated by 7.5 IU pregnant mare serum gonadotropin (PMSG) through an intraperito...

The Effect of Vitrification on Mouse Oocytes Apoptosis by Cryotop Method

Background: Oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. The present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by Cryotop method. Method: A total of 200 germinal vesicle (...

Accuracy of a rapid carbohydrate oxidation microtube method for identification of nonfermentative gram-negative bacilli.

A rapid carbohydrate oxidation microtube system (Carr Microbiologicals, Wichita, Kans.), designed for detecting the saccharolytic activity of gram-negative, nonfermenting bacilli, was evaluated and compared with the conventional oxidation-fermentation method. The oxidation of glucose, maltose, lactose, and xylose was tested with 430 strains of Pseudomonas, Acinetobacter, Achromobacter, Alcalige...